The Maglemosian skeleton from Koelbjerg, Denmark revisited

identifying sex and provenance

Authors

  • Jesper Hansen
  • Hans Christian Petersen
  • Karin Margarita Frei
  • Patrice Courtaud
  • Anne-marie Tillier
  • Anders Fischer
  • Morten E. Allentoft

DOI:

https://doi.org/10.1080/21662282.2017.1381418

Keywords:

Koelbjerg, skeleton, Maglemosian, Mesolithic, aDNA, haplogroup, osteology, strontium isotopes, sex identification

Abstract

The Koelbjerg individual, dated c. 8500 cal BC, represents the earliest human skeletal remains described from Scandinavia. Based on ancient DNA, strontium isotope and statistical anthropological analyses the individual’s sex, haplogroup and geographical provenance are here analysed and discussed. In contrast to previous claims, our genetic and anthropological analyses show that this individual was a male. Additionally, the strontium isotope ratio of one of his first molars indicates that he most likely grew up locally.

Author Biographies

Jesper Hansen

Archaeological Department, Odense City Museums, Odense C, Denmark

Hans Christian Petersen

IMADA, University of Southern Denmark, Odense M, Denmark

Karin Margarita Frei

Environmental Archaeology and Materials Science, National Museum of Denmark, Kongens Lyngby, Denmark

Patrice Courtaud

UMR 5199 PACEA - Anthropologie des Populations Passées et Présentes, Université de Bordeaux, Pessac Cedex, France

Anne-marie Tillier

UMR 5199 PACEA - Anthropologie des Populations Passées et Présentes, Université de Bordeaux, Pessac Cedex, France

Anders Fischer

SeaLand Archaeology, Kalundborg, Denmark

Morten E. Allentoft

Centre for GeoGenetics, Natural History Museum, University of Copenhagen, Copenhagen K, Denmark

References

ADBOU, 2016. ADBOU Age estimation, version 2.1.046. Available from: http://math.mercyhurst.edu/~sousley/Software/ [Accessed 27 August 2017].

Ahlström, T., 2013. Setting her even straighter: further notes on the osteology and necrodynamics of the Mesolithic burial from Barum, Scania, Sweden. In: S. Bergerbrant and S. Sabatini, eds. Counterpoint. Essays in archaeology and heritage studies in honour of professor Kristian Kristiansen BAR International Series 2508. Oxford, NY: Oxford University Press, 285-293.

Allentoft, M.E., et al., 2012. The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils. Proceedings of the Royal Society B, 279, 4724-4733. https://doi.org/10.1098/rspb.2012.1745

Allentoft, M.E., et al., 2015. Population genomics of Bronze Age Eurasia. Nature, 522, 167-172. https://doi.org/10.1038/nature14507

Bennike, P., 1986. Kvinden fra Koelbjerg på Fyn. Fynske Minder, 1986, 13-27.

Bennike, P., 1999. The early Neolithic Danish bog finds: a strange group of people. In: B. Coles, J. Coles, and M.S. Jørgensen, eds. Bog bodies, sacred sites and wetland archaeology. Exeter: University of Exeter, 27-32.

Boldsen, J.L. 2002. Transition analysis: a new method for estimating age from skeletons. In: R.D. Hoppa, et al., eds. Paleodemography: age distributions from skeletal samples. Cambridge: Cambridge University Press, 73-106. https://doi.org/10.1017/CBO9780511542428.005

Brandt, G., et al., 2013. Ancient DNA reveals key stages in the formation of Central European mitochondrial genetic diversity. Science, 342, 257-261. https://doi.org/10.1126/science.1241844

Bröste, K. and Balslev-Jørgensen, J.B., 1956. Prehistoric man in Denmark, volume 1 - Stone and Bronze Ages, part one. Copenhagen: Munksgaard.

Bröste, K. and Fischer-Møller, K., 1943. Koelbjerg Skelettet. Et Fund fra tidlig Maglemosetid [Le squelette de Koelbjerg]. Aarbøger for Nordisk Oldkyndighed og Historie, 1943, 211-231 [XXXI-XXXIII].

Bruzek, J., 2002. A method for visual determination of sex, using the human hip bone. American Journal of Physical Anthropology, 117, 157-168. https://doi.org/10.1002/(ISSN)1096-8644

Bruzek, J. and Murail, P., 2006. Methodology and reliability of sex determination from the skeleton. In: A. Schmitt, E. Cunha, and J. Pinheiro, eds. Forensic anthropology and medicine: complementary sciences from recovery to cause of death. New York: Springer, 225-242. https://doi.org/10.1007/978-1-59745-099-7_9

Bruzek, J. et al., 2017. Validation and reliability of the sex estimation of the human os coxae using freely available DSP2 software for bioarchaeology and forensic anthropology. American Journal of Physical Anthropology, 164, 440-449. Software available from: http://projets.pacea.u-bordeaux.fr/logiciel/DSP2/dsp2.html [Accessed 27 August 2017]. ttps://doi.org/10.1002/ajpa.23282

Constandse-Westermann, T.S., 1974. L'homme Mésolithique de nord-ouest de l'Europe. Distances biologiques, considérations génétiques. Bulletins et Mémoires de la Société d'Anthropologie de Paris, série XIII, 1, 173-199. https://doi.org/10.3406/bmsap.1974.2248

Damgaard, P.B., et al., 2015. Improving access to endogenous DNA in ancient bones and teeth. Scientific Reports, 5. https://doi.org/10.1038/srep11184

Donlon, D., 1993. Imbalance in the sex ratio in collections of Australian Aboriginal skeletal remains. In: H. du Cros and L. Smith, eds. Women in Archaeology: A Feminist Critique. Occasional Papers in Prehistory. no. 23. Canberra: Australian National University, 98-103.

Evans, J.A., et al., 2010. Spatial variations in biosphere 87Sr/86Sr in Britain. Journal of the Geological Society, 167, 1-4. https://doi.org/10.1144/0016-76492009-090

Fischer, A., 1996. At the border of human habitat. The Late Palaeolithic and Early Mesolithic in Scandinavia. In: L. Larsson, ed. The earliest settlement of Scandinavia and its relationship with neighbouring areas. Acta Archaeologica Lundensia, Series in 8, No. 24. Stockholm: Almquist & Wiksell, 157-176.

Fischer, A., et al., 2007. Coast- inland mobility and diet in the Danish Mesolithic and Neolithic: evidence from stable isotope values of humans and dogs. Journal of Archaeological Science, 34, 2125-2150. https://doi.org/10.1016/j.jas.2007.02.028

Frei, K.M., 2013. Exploring the potential of the strontium isotope tracing system in Denmark. Danish Journal of Archaeology, 2, 113-122. https://doi.org/10.1080/21662282.2012.760889

Frei, K.M. and Frei, R., 2011. The geographic distribution of strontium isotopes in Danish surface waters - a base for provenance studies in archaeology, hydrology and agriculture. Applied Geochemistry, 26, 326-340. https://doi.org/10.1016/j.apgeochem.2010.12.006

Frei, K.M. and Price, T.D., 2012. Strontium isotopes and human mobility in prehistoric Denmark. Archaeological and Anthropological Sciences, 4, 103-114. https://doi.org/10.1007/s12520-011-0087-7

Frei, K.M., et al., 2015. Tracing the dynamic life story of a Bronze Age Female. Scientific Reports, 5, 10431. https://doi.org/10.1038/srep10431

Fu, Q., et al., 2013. DNA analysis of an early modern human from Tianyuan Cave, China. Proceedings of the National Academy of Sciences of the United States of America, 110, 2223-2227. https://doi.org/10.1073/pnas.1221359110

Fund og Fortidsminder, Available from: http://www.kulturarv.dk/fundogfortidsminder/ [Accessed 29 August 2017].

Gambier, D., et al., 2006. Révision du sexe et de l'âge au décès des fossiles de Cro-Magnon (Dordogne, France) à partir de l'os coxal. Compte Rendu Palevol, 5, 735-741. https://doi.org/10.1016/j.crpv.2005.12.011

Gejvall, N.-G., 1970. The Fisherman from Barum - mother of several children! Fornvännen, 65, 281-289.

Gerasomova, M.M. and Pežemskij, D.V., 2005. Mezolitičeskij Čelovek iz Pesčanicy: kompleksnyj Antropologičeskij Analiz. Moscow: Rossiskij Akademija, Nauk.

Haak, W., et al., 2008. Ancient DNA, Strontium isotopes, and osteological analyses shed light on social and kinship organization of the Later Stone Age. Proceedings of the National Academy of Sciences of the United States of America, 105, 18226-18231. https://doi.org/10.1073/pnas.0807592105

Haglund, W.D. and Sorg, M.H., 2002. Human remains in water environments. In: W.D. Haglund and M.H. Sorg, eds. Advances in forensic taphonomy. Method. Washington, DC: Theory and Archaeological Perspectives, 201-218. https://doi.org/10.1201/9781420058352-13

Henke, W., 1987. Application of multivariate statistics to the problems of Upper Palaeolithic and Mesolithic samples. Human Evolution, 2, 149-167. https://doi.org/10.1007/BF02436403

Henriksen, M.B. and Hansen, J., 2017. En 10.000 årig skifter køn - et portræt af jubilaren fra Koelbjerg. Odense Bys Museers Årbog, 2017, 70-89.

Hillson, S., 1996. Dental anthropology. Cambridge: Cambridge University Press. ttps://doi.org/10.1017/CBO9781139170697

Horwitz, E.P., Chiarizia, R., and Dietz, M.L., 1992. A novel strontium-selective extraction chromatographic resin. Solvent Extraction and Ion Exchange, 10, 313-336. https://doi.org/10.1080/07366299208918107

Jacobs, K.H., 1985. Evolution in the postcranial skeleton of Late Glacial and early Postglacial European hominids. Zeitschrift für Morphologie und Anthropologie, 75, 307-326. https://doi.org/10.1515/zava.1985.75.2.307

Johansson, A.D., 1990. Barmosegruppen. Præboreale bopladser i Sydsjælland. Aarhus: Aarhus Universitetsforlag.

Li, H. and Durbin, R., 2009. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics, 25, 1754-1760. https://doi.org/10.1093/bioinformatics/btp324

Li, H., et al., 2009. The sequence alignment/map format and SAM tools. Bioinformatics, 25, 2078-2079. https://doi.org/10.1093/bioinformatics/btp352

Martin, R. and Saller, K., 1957. Lehrbuch der Anthropologie in systematischer Darstellung. Band I, 3. Aufl. Stuttgart: Gustav Fischer Verlag.

Meyer, M. and Kircher, M., 2010. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harbor Protocols, 2010, pdb. prot5448. https://doi.org/10.1101/pdb.prot5448

Milner, G.R. and Boldsen, J.L., 2012. Transition analysis: a validation study with known-age modern American skeletons. American Journal of Physical Anthropology, 148, 98- 110. https://doi.org/10.1002/ajpa.v148.1

Montgomery, J., 2010. Passports from the past: investigating human dispersals using strontium isotope analysis of tooth enamel. Annals of Human Biology, 37, 325-346. https://doi.org/10.3109/03014461003649297

Moree, J.M. and Sier, M.M., eds., 2015. Interdisciplinary Archaeological Research Programme Maasvlakte 2, Rotterdam. Part 1: twenty metres deep! The Mesolithic period at the Yangtze Harbour site - Rotterdam Maasvlakte, The Netherlands. Early Holocene landscape development and habitation. BOORrapporten, 566. Rotterdam: Bureau Oudheidkundig Onderzoek Rotterdam.

Murail, P., et al., 2005. DSP - a tool for probabilistic sex diagnosis using worldwide variability in hip-bone measurements. Bulletins et Mémoires de la Société d'Anthropologie de Paris, 17 (n.s.), 167-176.

Newell, R.R., Constandse-Westermann, T.S., and Meiklejohn, C., 1979. The skeletal remains of Mesolithic man in Western Europe: an evaluative catalogue. Journal of Human Evolution, 8, 1-228.

Pearson, K., 1899. Mathematical contributions to the theory of evolution. - V. On the reconstruction of the stature of prehistoric races. Philosophical Transactions of the Royal Society of London, Series A, 192, 169-244. https://doi.org/10.1098/rsta.1899.0004

Petersen, H.C., 1998. Nyt lys over skelettet fra Koelbjerg: et menneske fra tidlig Maglemosetid. In: 3rd Nordic seminar on biological anthropology, clara lachmann symposium, Copenhagen: Department of Forensic Medicine, University of Copenhagen.

Petersen, H.C., 2004. Porotic hyperostosis and other new findings concerning the early Mesolithic skeleton from Koelbjerg, the oldest human remains from Denmark (abstract). Bulletins et Mémoires de la Société d'Anthropologie de Paris, 15 (n.s.), 302.

Petersen, H.C., Courtaud, P., and Tillier, A.-M., 2009. Personal identification in the Mesolithic - sex determination of a case from Denmark. In: First baltic bioarchaeology meeting - "Bioarchaeology and Forensic Anthropology", 24- 27 August. Vilnius: Institute of Forensic Medicine, Mykolas Romeris University & Department of Anatomy, Histology and Anthropology, Faculty of Medicine, Vilnius University.

Phenice, T.W., 1969. A newly developed visual method of sexing the os pubis. American Journal of Physical Anthropology, 30, 297-301. https://doi.org/10.1002/(ISSN)1096-8644

Posth, C., et al., 2016. Pleistocene mitochondrial genomes suggest a single major dispersal of non-Africans and a Late Glacial population turnover in Europe. Current Biology, 26, 827-833. https://doi.org/10.1016/j.cub.2016.01.037

Price, T.D., et al., 2017. Multi-isotope proveniencing of human remains from a Bronze Age battlefield in the Tollense Valley in northeast Germany. Archaeological and Anthropological Sciences. https://doi.org/10.1007/s12520-017-0529-y

Ravn, M., 2010. Burials in bogs - Bronze and Early Iron Age bog bodies from Denmark. Acta Archaeologica, 81-1, 112- 123. https://doi.org/10.1111/j.1600-0390.2010.00307.x

Rogers, T.L., 1999. A visual method of determining the sex of skeletal remains using the distal humerus. Journal of Forensic Sciences, 44, 57-60. https://doi.org/10.1520/JFS14411J

Rösing, F.W., et al., 2007. Recommendations for the forensic diagnosis of sex and age from skeletons. HOMO - Journal of Comparative Human Biology, 58, 75-89. https://doi.org/10.1016/j.jchb.2005.07.002

Schubert, M., Lindgreen, S., and Orlando, L., 2016. AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Research Notes, 9, 1. https://doi.org/10.1186/s13104-016-1900-2

Sjøvold, T., 1990. Estimation of stature from long bones utilizing the line of organic correlation. Human Evolution, 5, 431-447. https://doi.org/10.1007/BF02435593

Skoglund, P., et al., 2013. Accurate sex identification of ancient human remains using DNA shotgun sequencing. Journal of Archaeological Science, 40, 4477-4482. https://doi.org/10.1016/j.jas.2013.07.004

Sommer, M., 2007. Bones and Ochre. The curious afterlife of the Red Lady of Paviland. Cambridge, MA: Harvard University Press.

Stewart, T.D., 1970. Identification of the scars of parturition in the skeletal remains of females. In: T.D. Stewart, ed. Personal identification in mass disasters. Washington, DC: Smithsonian Press, 127-135. https://doi.org/10.5479/sil.30678.39088001440254

Tauber, H., 1986. Koelbjerg-kvindens alder og ernæring. Fynske Minder, 1986, 28-33.

Troels-Smith, J., 1943. Geologisk Datering af Koelbjerg- Skelettet [Datation géologique du squelette de Koelbjerg]. Aarbøger for Nordisk Oldkyndighed og Historie, 1943, 232- 238 [XXXIV-XXXVI].

van der Sanden, W., 1996. Through nature to eternity. The bog bodies of Northwest Europe. Amsterdam: Batavian Lion International.

Villotte, S., 2009. Enthésopathies et activités des hommes préhistoriques. Recherche méthodologique et application aux fossiles européens du Paléolithique supérieur et du Mésolithique. BAR International Series 1992. Oxford: BAR (British Archaeological Reports). https://doi.org/10.30861/9781407305264

Voerkelius, S., et al., 2010. Strontium isotopic signatures of natural mineral waters, the reference to a simple geological map and its potential for authentication of food. Food Chemistry, 118, 933-940. https://doi.org/10.1016/j.foodchem.2009.04.125

Walker, P.W., 1995. Problems of preservation and sexism in sexing: some lessons from historical collections for palaeodemographers. In: S.R. Saunders and A. Herring, eds. Grave reflections. portraying the past through cemetery studies. Toronto: Canadian Scholars' Press, 31-47.

Weiss, K.M., 1972. On the systematic bias in skeletal aging. American Journal of Physical Anthropology, 37, 239-250. https://doi.org/10.1002/ajpa.1330370208

Weyrich, L.S., et al., 2017. Neanderthal behaviour, diet, and disease inferred from ancient DNA in dental calculus. Nature Research Letter. https://doi.org/10.1038/nature21674

Willerslev, E. and Cooper, A., 2005. Ancient DNA. Proceedings of the Royal Society B-Biological Sciences, 272, 3-16. https://doi.org/10.1098/rspb.2004.2813

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2017-05-01

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